Mobilization of human
CD34+CD133+ and CD34+CD133− stem
cells in vivo by consumption of an extract from Aphanizomenon flos-aquae—related
to modulation of CXCR4 expression by an L-selectin ligand?☆
, Aaron N. Hartb, Lue A.M. Zaskeb, Christian Drapeauc, Niraj Guptad, David J. Schaeffere, J. Alex Cruickshankb
The goal of this study was to
evaluate effects on human stem cells in vitro and in vivo of an extract from
the edible cyanobacterium Aphanizomenon flos-aquae (AFA) enriched for a
novel ligand for human CD62L (L-selectin).
Experimental
approach
Ligands for CD62L provide a mechanism for stem cell mobilization in conjunction with down-regulation of the CXCR4 chemokine receptor for stromal derived factor 1. Affinity immunoprecipitation was used to identify a novel ligand for CD62L from a water extract from AFA. The effects of AFA water extract on CD62L binding and CXCR4 expression was tested in vitro using human bone marrow CD34+ cells and the two progenitor cell lines, KG1a and K562. A double-blind randomized crossover study involving 12 healthy subjects evaluated the effects of consumption on stem cell mobilization in vivo.
Ligands for CD62L provide a mechanism for stem cell mobilization in conjunction with down-regulation of the CXCR4 chemokine receptor for stromal derived factor 1. Affinity immunoprecipitation was used to identify a novel ligand for CD62L from a water extract from AFA. The effects of AFA water extract on CD62L binding and CXCR4 expression was tested in vitro using human bone marrow CD34+ cells and the two progenitor cell lines, KG1a and K562. A double-blind randomized crossover study involving 12 healthy subjects evaluated the effects of consumption on stem cell mobilization in vivo.
Results
An AFA extract rich in the CD62L ligand reduced the fucoidan-mediated externalization of the CXCR4 chemokine receptor on bone marrow CD34+ cells by 30% and the CD62L+ CD34+ cell line KG1A by 50% but did not alter the CXCR4 expression levels on the CD34− cell line K562. A transient, 18% increase in numbers of circulating CD34+ stem cells maximized 1 hour after consumption (P<.0003). When 3 noncompliant volunteers were removed from analysis, the increase in CD34+ cells was 25% (P<.0001).
An AFA extract rich in the CD62L ligand reduced the fucoidan-mediated externalization of the CXCR4 chemokine receptor on bone marrow CD34+ cells by 30% and the CD62L+ CD34+ cell line KG1A by 50% but did not alter the CXCR4 expression levels on the CD34− cell line K562. A transient, 18% increase in numbers of circulating CD34+ stem cells maximized 1 hour after consumption (P<.0003). When 3 noncompliant volunteers were removed from analysis, the increase in CD34+ cells was 25% (P<.0001).
Conclusion
AFA water extract contains a novel
ligand for CD62L. It modulates CXCR4 expression on CD34+ bone marrow cells in
vitro and triggers the mobilization of CD34+ CD133+ and CD34+ CD133− cells in
vivo.
The coauthor C. Drapeau is Chief
Science Officer for StemTech
Health Sciences, which distributes the product StemEnhance, used for the in vivo part of
this paper. No other authors have any commercial interest in the subject matter.
Abbreviations: AFA, Aphanizomenon flos-aquae, PBMC, Peripheral blood mononuclear cells, PMN, Polymorph-nucleated cells
Abbreviations: AFA, Aphanizomenon flos-aquae, PBMC, Peripheral blood mononuclear cells, PMN, Polymorph-nucleated cells
Keywords: L-selectin,
Ligand, Human, Adult stem cell,
CD34, CD133, KG1a, K562, Bone marrow,
Mobilization,
Blue-green algae,
Cyanobacteria,
Aphanizomenon,
In vivo, In vitro
National Library Of Medicine’s PubMed
http://www.ncbi.nlm.nih.gov/pubmed/17765649?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
Interview Platform Magazine
Interview Platform Magazine
No comments:
Post a Comment